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Active Arl1 GTP [26924]
[30 µL]
Catalog Number: 26924 |
Gene Symbol: Arl1 |
Description: Anti active Arl1 Mouse Monoclonal Antibody |
Background: Arl1 (Arf-like 1) localizes to the Golgi complex, where it is believed to recruit effector proteins to the trans-Golgi network. Golgi-localized proteins golgin-97 and golgin-245 have been shown as Arl1 effectors. Additional Arl1 effectors include the GARP (Golgi-associated retrograde protein)/VFT (Vps53) vesicle-tethering complex and Arfaptin 2. In Drosophila zygotes, mutation of Arl1 is lethal. |
Immunogen: Recombinant Arl1 protein (active form) |
Applications: IP, IHC, IF |
Recommended Dilutions: 1 µg for 1~2 mg total cellular proteins for IP 1:50~100 dilution for IHC, IF |
Concentration: 1 mg/ml |
Host: mouse |
Format: Liquid |
Clonality: Monoclonal |
Isotype: IgG |
Purity: Purified from ascites |
Storage buffer: |
Preservative: no |
Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
Species Reactivity: Arl1 antibody recognizes active Arl1-GTP from vertebrates. |
Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles |
Immunoprecipitation/Western blot: |
Arl1 Activation Assay. Purified GST-tagged Arl1 proteins (Cat. #10136) were immunoprecipitated with the anti active Arl1 monoclonal antibody (Cat. # 26924) after treated with GDP (lane 1) or GTPγS (lane 2), and was blotted with anti Arl1 monoclonal antibody (Cat. #26056). Arl1 protein input control is shown in the bottom panel. |
Related Products | |||
Catalog# | Name | Size | Price |
26924 | Active Arl1 GTP Monoclonal Antibody | 30 μL | ¥4800 |
82901 | Arl1 Activation Assay Kit | 20 assay | ¥6800 |
26056 | Anti Arl1 Mouse Monoclonal Antibody | 100 μL | ¥2800 |