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Anti- ERBB2(V777A) mouse monoclonal Antibody

價(jià)格¥4800.00
品牌NewEast Biosciences   
產(chǎn)地中國(guó) 武漢
貨號(hào)26080
規(guī)格100μl
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  • ErbB2(V777A) [26080]
    [100 µL]

     

    Catalog Number:  26080
    Gene Symbol:  ERBB2/HER2/Neu
    Description:   Anti ErbB2(V777A) mouse monoclonal Antibody
    Background:   The ErbB family is composed of four plasma membrane-bound receptor tyrosine kinases. Amplification or over-expression of the ERBB2 gene occurs in approximately 30% of breast cancers. HER2 is encoded by ERBB2, a known proto-oncogene located at the long arm of human chromosome 17(17q21-q22). HER2 proteins have been shown to form clusters in cell membranes that may play a role in tumorigenesis. Mutations of ErbB2 protein, including V777A, have been indicated in human cancers.
    Immunogen:   A synthetic peptide surround the 777A codon of ErbB2 protein.
    Tested Applications:   ELISA, WB, IF, IHC
    Recommended Dilutions:   
    ELISA: 1:500-1:5000 
    WB: 1:200-1:2000 
    IF: 1:100 
    IHC: 1:50-1:100
    Concentration:   0.2 mg/ml
    Host:   mouse
    Format:   Liquid
    Clonality:   Monoclonal
    Isotype:   
    Purity:   Purified from ascites
    Storage buffer:
    Preservative:  no
    Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
    Species Reactivity:  Recognizes V777A mutant but not wild type ErbB2 protein, of vertebrates.
    Storage Conditions:  Store at -20°C. Avoid freeze / thaw cycles.
     
    Immunofluorescence:  
    Immunofluorescence of cells expressing ErbB2 proteins with Anti ErbB2(V777A) antibody. HEK293T cells were transfected with pCDNA3-GFP ErbB2 WT plasmid (left column) or pCDNA3-GFP ErbB2(V777A) plasmid (right column), then fixed and stained with Anti ErbB2(V777A) monoclonal antibody (Cat. #26080).

     

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    21202 Anti ERBB2 Rabbit Polyclonal Antibody  100 μL  ¥2600   
    26080 Anti ERBB2(V777A) mouse monoclonal Antibody  100 μL  ¥4800   
    26127 Anti ERBB2 mouse monoclonal Antibody  100 μL  ¥2800  
    26382 Anti ERBB2(D769Y) Mouse Monoclonal Antibody  100 μL  ¥4800
    26381 Anti ERBB2(D769H) Mouse Monoclonal Antibody  100 μL  ¥4800

     

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