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Anti- GRB7 Mouse Monoclonal Antibody

價(jià)格1600.00
品牌NewEast Biosciences   
產(chǎn)地中國(guó)武漢
貨號(hào)26133
規(guī)格100μl
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  • GRB7 [26133]
    [100 µL]

     

    Catalog Number:  26133
    Gene Symbol:  GRB7
    Description:   anti GRB7 Mouse Monoclonal Antibody
    Background:   Grb7 is an adaptor protein that interacts with epidermal growth factor receptor (EGFR) and ephrin receptors. It plays a role in the integrin signaling pathway and cell migration by binding with focal adhesion kinase (FAK). Grb7 gene is commonly co-amplified (present in excess copies) in breast cancers. Grb7 is over-expressed in testicular germ cell tumors, esophageal cancers, and gastric cancers. There are clues that it involved in cell migration and metastatic progression of human esophageal carcinomas.
    Immunogen:   Purified GRB7 protein, human origin.
    Applications:   ELISA, WB, IHC
    Recommended Dilutions:   
    ELISA: 1:1000-1:5000 
    WB: 1:100-1:1000 
    IHC: 1:50-1:200
    Concentration:    0.5 mg/ml
    Host:   Mouse
    Format:   Liquid
    Clonality:   Monoclonal
    Isotype:   
    Purity:   Purified from ascites
    Storage buffer:
    Preservative:  no
    Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
    Species Reactivity:  Recognizes GRB7 of vertebrates.
    Storage Conditions:   Store at -20°C. Avoid freeze / thaw cycles
     
    Western blot:  
    Western blot analysis of recombinant GRB7 protein. Purified His-tagged GRB7 protein was blotted with anti GRB7 mouse monoclonal antibody (Cat. #26133).
    Immunohistochemistry:  
    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with anti GRB7 monoclonal antibody (Cat. #26133). Tissue samples were fixed with formaldehyde and blocked with 1% serum for 15 min at 37 °C. Antigen retrieval was by heat mediation in citrate buffer (pH6). Samples were then incubated with primary antibody (1:50 dilution) overnight at 4°C. A HRP-conjugated Goat anti-mouse IgG (1:50 dilution) was used as secondary antibody.

     

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